Abstract： Objective To explore the curative effects of Lamivudine combined with IFNα-1b in treatment of chronic hepatitis B. Methods 120 patients with chronic hepatitis B were divided into 3 groups. Patients in group A were treated with IFNα-2b 3 times a week for 26 weeks. Patients in group B were treated with Lamivudine one time a day for 52 weeks. Patients in group C were treated with IFNα-1b and Lamivudine symphysially. The serum indexes were observed and the clinical effects were evaluated. Results The ALT and AST levels in 3 groups were all decreased significantly(P ＜0. 01) after treatment. However,the ALT and AST in group C was lower than that of the other 2 groups(P ＜0. 05). Also there was a statistical difference(P ＜0. 05) between the effective rate of the group C and that of other 2 groups. Conclusion Treating chronic hepatitis B with Lamivudine and IFNα-1b federatively was more effective than using a single medicine.