Abstract： Objective To evaluate the effects of p53 combined with elenlene and 5-Fu infusion via portal vein for preventing recurrence of small liver cancer after operation.Methods Five male patients with small fiver cancer which have been performed operation(2 cases)or combine interventional procedures(3 cases).A 4F catheter was inserted into portal vein under guiding by B type ultrasound and fluoroscopy.The drugs infused into portal vein in-cluded recombinant human Ad-p53 injection,elemene emulsion,and 5Fu.After finished the drugs infusion plan,the catheter was removed and puncturing duct of the live was occluded by TH glue.Results Five patients received the treatment according to treating plan.Portal vein catheter insertion and remove was finished smoothly.No complications such as portal vein thrombosis,bleeding of live puncturing duct,catheters abnormal location were found.Side effects of p53 gone was fever as 39～40℃ or 1 to 3 hours.Elemene and 5-Fu injection has micro side effects.Five patients were followed up for 5 t0 16 months(average 8.5 months).There were no tumor recurrence evidence provided by radi-ology and hemotology during following time.Conclusion Primary results suggest that p53 infusion via portal vein may inhibit and treat fiver camcer recurrence.Control stndy and long time following is needed.