Abstract： Objective Through the investigation and analysis,Find the influencing factors of nosocomial infection,seek to control the incidence of nosocomial infection methods and countermeasures.Methods Use retrospective study to find 293 infection cases,and 286 normal cases,then process Logistic regression analysis of the second category.Results The influencing factors of nosocomial infection are:the number of days hospitalized,ICU days,age,blood transfusion,the number of days serious ill,the sections change times.Conclusion In order to control the incidence of nosocomial infection,focus on the crowd of high-risk factors,bring in the performance appraisal,establish the HIS to realtime monitor.