The empirical study on the effect of CIK cell on apoptosis of Lewis lung cancer cell
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
- Issue:
- 12
- DOI:
- 10.3760/cma.j.issn.1008-6706.2008.12.004
- Key Word:
- CIK细胞;Lewis肺癌;细胞凋亡;Fas/FasL;Cytokine induced killer;Lewis lung carcinoma;Apoptosis;Fas/FasL
Abstract： Objective To investigate the inhibitory effects of CIK on the proliferation of Lewis lung carcinoma cell line and the growth of transplanted Lewis lung carcinoma in C57BL/6N mice in vivo.Methods CIK cells were induced by culturing PBMC with regular method.The proliferation of Lewis lung carcinoma cells was measured by MTT assay.Uhramicrostrueture of Lewis lung carcinoma cells was observed under a transmission electron microscope.Flowcytometric analysis was used to detect cell apoptosis.Ultrastructural observation expressions of FasL were individually determined by MTT and immunocytochemistry(ICC) analysis.Results Electron microscopic observations sbowed that CIK cells could induce the hepatoma cells to apoptosis.Flow cytometric analysis demonstrated that apoptosis cells of Lewis lung carcinoma were increased in CIK group compared with those in the control group.FasL expression on CIK increased.Cytotoxieity was blocked after addition of anti-FasmAb.Conclusion CIK has inhibitory effect on Lewis lung carcinoma cells both in vitro and in vivo.CIK cells can induce the apoptosis of Lewis lung carcinoraa cells.and Fas/FasL pathway plays an important role in apoptosis of Lewis lung carcinoma cells by CIK.
- [1]李淑艳,王宏兰,任欢,等.CIK细胞与LAK细胞对Lewis肺癌小鼠的抑瘤作用[J].2003,(9).
- [2]Wells S,Leemhuis T,Negrin RS,etc.A phase I trial of autologous cytokine-induced killer cells for the treatment of relapsed Hodgkin disease and non-Hodgkin lymphoma.[J].2005,11(3).
- [3]C, Hoyle,C D, Bangs,P, Chang,etc.Expansion of Philadelphia chromosome-negative CD3(+)CD56(+) cytotoxic cells from chronic myeloid leukemia patients: in vitro and in vivo efficacy in severe combined immunodeficiency disease mice.[J].1998,92(9).
- [4]M, Hishii,J T, Kurnick,T, Ramirez-Montagut,etc.Studies of the mechanism of cytolysis by tumour-infiltrating lymphocytes.[J].1999,116(3).
- [5]I. G. H. Schmidt-Wolf,P. Lefterova,V. Johnston,etc.Propagation of large numbers of T cells with natural killer cell markers[J].1994,87(3).
- [6]Suradej, Hongeng,Sawang, Petvises,Surapon, Worapongpaiboon,etc.Generation of CD3+ CD56+ cytokine-induced killer cells and their in vitro cytotoxicity against pediatric cancer cells.[J].2003,77(2).
- [7]Schakowski F,Schottker B,Ziske C,etc.Interactions between dendritic cells and cytokine-induced killer cells lead to an activation of both populations.[J].2001,24(6).
- [8]I G, Schmidt-Wolf,P, Lefterova,B A, Mehta,etc.Phenotypic characterization and identification of effector cells involved in tumor cell recognition of cytokine-induced killer cells.[J].1993,21.
- [9]P H, Lu,R S, Negrin.A novel population of expanded human CD3+CD56+ cells derived from T cells with potent in vivo antitumor activity in mice with severe combined immunodeficiency.[J].1994,153(4).