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Glypican-3 expression in hepatocellular carcinoma by RT-PCR and SSCP
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.1008-6706.2007.10.003
- Key Word:
- Glypican-3基因;癌,肝细胞;突变
Abstract： 目的 观察Glypican-3基因在肝细胞癌中的表达和突变关系.方法 采用RT-PCR和SSCP分别检测48例肝细胞癌肿瘤组织、癌旁组织和正常肝组织中Glypican-3 mRNA的表达和基因突变.结果 Glypican-3 mRNA在肿瘤组织中的阳性表达率为77.1%,癌旁和正常肝组织中未见表达;Glypican-3表达与肝癌临床分期、细胞分化程度均有显著相关性(P＜0.05);肝细胞癌组织中未发现Glypican-3基因突变.结论 正常基因型Glypican-3在肝细胞癌中的过度表达可能是作为"安全卫士"来对抗癌基因的异常表达,从而发挥抑癌作用.
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