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The change of Bax/Bcl-2 on endotoxin-induced acute lung injury
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.1008-6706.2006.10.001
- Key Word:
- 大鼠;肺损伤,急性;细胞凋亡
Abstract： 目的 观察脂多糖(LPS)致大鼠急性肺损伤(ALI)后大鼠肺泡上皮细胞凋亡情况.方法 雄性Wistar大鼠200～250 g 60只,随机分为两组,分别为对照组和LPS组,每组30只.各组分别注射0.9%氯化钠(NS)或LPS 5 mg/kg 4 h后观察呼吸频率,行动脉血气分析;致死后检测肺组织湿/干(W/D)比值,检测肺泡上皮细胞B-细胞淋巴瘤/白血病-2(Bcl-2)、Bcl-2相关X蛋白(Bax)表达.结果 LPS组呼吸频率[(108±77)次/min]较对照组[(71±5)次/min]显著增快,且有明显的肺水肿和低氧血症.LPS组Bax蛋白表达阳性细胞率[(6.27 ± 0.84)%]与对照组[(0.48±0.52)%]相比显著增高,Bcl-2蛋白表达阳性细胞率与对照组比较差异无统计学意义.结论 促进细胞凋亡的Bax蛋白表达细胞显著增多,使Bax/Bcl-2细胞比例明显增加,可能参与了LPS诱发的ALI的发病机制.
- [1]翟诚顺,马中富.细胞凋亡与多器官功能障碍综合征的研究进展[J].2003,(10).
- [2]中华医学会呼吸病学分会.急性肺损伤/急性呼吸窘迫综合征的诊断标准（草案）[J].2000,(4).
- [3]C,MÜ,LLER-LEISSE B,etc.Computed Tomography and Histologic Results in the Early Stages of Endotoxin-Injured Pig Lungs as a Model for Adult Respiratory Distress Syndrome[J].1993,28(1).
- [4]Buchman TG,Karl IE,Hotchkiss RS,etc.Overexpression of Bcl-2 in transgenic mice decreases apoptosis and improves survival in sepsis.[J].1999,162(7).