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Effect evaluation of chocolate agar media made of horse, rabbit and sheep blood using for isolate haemophilus influenzae
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1008-6706.2006.05.004
- Key Word:
- 动物;血;巧克力琼脂培养基;嗜血菌,流感
Abstract： 目的比较马血、兔血、羊血巧克力琼脂培养基痰培养流感嗜血杆菌的检出率和生长情况.方法将送检的痰标本及流感嗜血杆菌标准菌株ATCC10211分别接种于马血、兔血、羊血巧克力琼脂培养基上,置烛缸中,36℃培养24 h,比较流感嗜血杆菌检出率及其生长情况.结果 (1)马血、兔血、羊血巧克力琼脂培养基痰培养流感嗜血杆菌的检出率分别为34.3%、35%、8.3%.(2)标准菌株ATCC10211在马血、兔血巧克力琼脂培养基上生长良好,菌落直径0.5～1.0 mm,而在羊血巧克力琼脂培养基上生长差,菌落大小如细粉末状,肉眼难见.结论痰培养流感嗜血杆菌使用马血、兔血巧克力琼脂培养基效果优于羊血巧克力琼脂培养基.
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