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Studying on the expression of P27, c-fos protein and DWI imaging after cerebral ischemia and reperfusion
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1008-6706.2006.03.003
- Key Word:
- 脑缺血;P27,c-fos蛋白;磁共振弥散加权成像
Abstract： 目的探讨大鼠脑缺血再灌注后脑内P27、c-fos蛋白阳性表达分布的规律,并应用磁共振弥散加权成像(magnetic resonance diffusion-weighted imaging,DWI)技术进行相关弥散加权成像研究.方法采用右侧大脑中动脉脑缺血模型,SD健康大鼠随机分为三组:A组,缺血30 min再灌注60 min;B组,缺血60 min再灌注60 min;C组,缺血60 min,非再通组.模型成功行DWI成像.免疫组化技术观察P27、c-fos蛋白脑组织内表达结果.结果缺血及再灌注各组中脑内P27蛋白未见免疫阳性细胞表达;在缺血侧皮层和基底节区可见c-fos阳性表达.15 min即可在DWI上观察到缺血区高信号表现.结论P27蛋白是一种非特异性的细胞周期素依赖酶抑制剂.它在大鼠脑组织中的表达低下,推测可能与神经元缺血后易引起程序性死亡的结局有关系.即早基因c-fos参与缺血性细胞损伤的发生.DWI对缺血有极好的成像敏感性.
- [1]Coates PW,Kuhn HG,Gage FH,etc.Survival and Differentiation of Adult Neuronal Progenitor Cells Transplanted to the Adult Brain[J].1995,92(25).
- [2]J, Nourse,E, Firpo,W M, Flanagan,etc.Interleukin-2-mediated elimination of the p27Kip1 cyclin-dependent kinase inhibitor prevented by rapamycin.[J].1994,372.
- [3]Linnik M. D.,Zobrist R. H.,Hatfield M. D..Evidence supporting a role for programmed cell death in focal cerebral ischemia in rats[J].1993.
- [4]Schreiber SS,Baudry M.Selective neuronal vulnerability in the hippocampus--a role for gene expression?[J].1995,18(10).
- [5]D, Peng,Z, Fan,Y, Lu,etc.Anti-epidermal growth factor receptor monoclonal antibody 225 up-regulates p27KIP1 and induces G1 arrest in prostatic cancer cell line DU145.[J].1996,56.
- [6]T, Malinski,F, Bailey,Z G, Zhang,etc.Nitric oxide measured by a porphyrinic microsensor in rat brain after transient middle cerebral artery occlusion.[J].1993,13.
- [7]Loubinoux I,Seylaz J,Borredon J,etc.Spreading of vasogenic edema and cytotoxic edema assessed by quantitative diffusion and T2 magnetic resonance imaging.[J].1997,28(2).
- [8]Nagasawa H.,Kogure K..Correlation between cerebral blood flow and histologic changes in a new rat model of middle cerebral artery occlusion[J].1989.
- [9]M. E. Moseley,Y. Cohen,J. Mintorovitch,etc.Early detection of regional cerebral ischemia in cats: Comparison of diffusion- and T2-weighted MRI and spectroscopy[J].1990,14(2).