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Expression of EBER-1 in fine-needle aspirate samples using in situ hybridization
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1008-6706.2005.06.004
- Key Word:
- 鼻咽肿瘤;淋巴结;肿瘤转移;原位杂交;疱疹病毒4型;人;活组织检查;细针;聚合酶链反应
Abstract： 目的探讨对颈部肿块细针吸取作EBER-1检测在诊断鼻咽癌转移中的意义.方法采用非同位素标记原位杂交(ISH)和聚合酶链反应技术(PCR)分别对27例颈肿块细针吸取涂片细胞和8例细针吸取液进行EB病毒小分子EBER-1和EB病毒DNA检测.结果鼻咽癌转移组23例原位杂交16例阳性(69.5%),非鼻咽癌转移组4例皆阴性.PCR 7例鼻咽癌转移者均阳性(100%),1例肺癌转移者阴性.结论应用ISH检测颈部肿块细针吸取涂片细胞中的EB病毒对诊断鼻咽癌具有重要的意义.
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