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Clinical significance of detecting CK19mRNA in the peripheral blood in non-small cell lung cancer patients
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1008-6706.2005.01.004
- Key Word:
- 癌,非小细胞肺;肿瘤转移;细胞角蛋白;聚合酶链反应
Abstract： 目的探讨非小细胞肺癌(non-small cell lung cancer,NSCLC)患者外周血CK19mRNA检测的临床意义.方法运用巢式逆转录聚合酶链反应方法(nested-reverse transcription polymerase chain reaction,Nested RT-PCR)检测78例NSCLC患者和32例肺良性疾病患者外周血CK19mRNA的表达,另选取20例健康成年人外周血作为对照.结果NSCLC患者外周血CK19mRNA表达阳性率为69.2%,肺良性疾病患者为6.3%(x2=36.014,P＜0.01),20例健康成年人无一表达.NSCLC患者外周血微转移与组织学类型、细胞分化程度及TNM分期均存在密切关系(P＜0.05或P＜0.01).结论CK19mRNA是一种诊断NSCLC外周血微转移的特异性标记物,Nested RT-PCR方法是检测外周血CK19mRNA表达的灵敏可靠技术,NSCLC微转移的诊断对判断病程发展和指导临床治疗具有重要意义.
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