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Experiment study on XRCC1 and DNA fragmentation after cerebral ischemia/reperfusion of renovascular hypertensive rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1008-6706.2004.03.002
- Key Word:
- 肾性高血压大鼠;局灶脑缺血;DNA片段化损伤;凋亡
Abstract： 目的观察肾性高血压大鼠局灶脑缺血再灌注后DNA修复蛋白X线修复交叉互补组1(XRCC1)蛋白的表达及与DNA片段化损伤的关系.方法采用双肾双夹法建立肾性高血压大鼠模型,在此基础上采用线栓法制作大脑中动脉闭塞模型,采用免疫组织化学方法观察假手术组和缺血再灌注后3 h、6 h、12 h、24 h XRCC1蛋白表达,采用TUNEL方法检测DNA片段化损伤.结果免疫组织化学染色显示,假手术组大脑皮质和海马区出现大量XRCC1表达的阳性细胞,再灌注3 h缺血区皮质和海马CA1区XRCC1表达阳性细胞减少,即缺血区皮质和海马CA1区XRCC1灰度值升高,并一直持续到缺血再灌注24 h.缺血再灌注后3 h、6 h、12 h和24 h与假手术组相比差异有显著意义(P＜0.05).TUNEL染色显示,假手术组大脑皮质和海马CA1区未见阳性细胞,再灌注24 h缺血区皮质和海马CA1区可见较多阳性细胞.结论脑缺血再灌注后XRCC1蛋白免疫活性下降和DNA修复机制的失败与DNA的片段化损伤及凋亡的发生有关.
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