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A study on apoptosis in neonatal infants with hypoxic ischemic encephalopathy
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1008-6706.2004.03.001
- Key Word:
- 脑缺氧;脑缺血;婴儿/新生儿;脱噬作用
Abstract： 目的探讨因患缺氧缺血性脑病(hypoxic ischemic cncephalopath,HIE)死亡新生儿的脑组织是否存在细胞凋亡情况.方法收集因HIE死亡新生儿5例(HIE组)及非因HIE死亡新生儿2例(非HIE组),以肺活检穿刺针自前囟穿刺取脑组织,通过HE染色、原位末端脱氧核苷酸转移酶标记法(TUNEL)及电镜等方法,观察脑细胞病理形态变化,计数单位面积下凋亡阳性细胞数.结果HIE组新生儿脑细胞在光镜及电镜下观察可见较多凋亡细胞及坏死细胞,TUNEL染色凋亡细胞阳性率平均约10.90个/mm2,非HIE组新生儿脑细胞少见凋亡,阳性率约3.50个/mm2,电镜下细胞结构保存完整.结论患HIE死亡新生儿脑细胞存在凋亡,从在体研究方面证实细胞凋亡参与新生儿HIE发病机制.
- [1]蒋犁,汤云珍,黄晓明.缺氧缺血大鼠脑细胞凋亡的研究[J].1998,(8).
- [2]宋薇薇,杜丽敏,尚涛,等.宫内窒息后新生鼠的状态与脑损伤关系的研究[J].2002,(4).
- [3]Mehmet H,Yue X,Azzopardi DV,etc.Apoptosis in the brains of infants suffering intrauterine cerebral injury.[J].1997,42(5).
- [4]Aito H,Aalto KT,Raivio KO.Biphasic ATP depletion caused by transient oxidative exposure is associated with apoptotic cell death in rat embryonal cortical neurons.[J].2002,52(1).
- [5]I E, Hill,J P, MacManus,I, Rasquinha,etc.DNA fragmentation indicative of apoptosis following unilateral cerebral hypoxia-ischemia in the neonatal rat.[J].1995,676(2).