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Cytotoxicity of dendritic cell to tumor cell lines
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
- Issue:
- 8
- DOI:
- 10.3760/cma.j.issn.1008-6706.2002.08.001
- Key Word:
- 树突状细胞;IFN-γ;LPS;肿瘤杀伤活性
Abstract： 目的对比分析干扰素-γ(Interferon-γ,IFN-γ)或脂多糖(lipoplysaccharide,LPS)刺激后的树突状细胞(dendritic cell,DC)对肿瘤细胞杀伤活性的差异.方法分离健康供者外周血单核细胞,用粒单细胞集落刺激因子和白介素-4诱导为DC.于培养液中加入LPS或IFN-γ培养12 h,作为LPS激活的DC(LPS-DC)及IFN-γ激活的DC(IFN-DC).用流式细胞仪检测DC表面共刺激分子的改变,以明确LPS或IFN-γ对DC的不同刺激作用;同时,以恶性血液病细胞株HL-60、Jurkat及Daudi为靶细胞,用不同效靶比与DC共同培养18 h,采用51Cr释放试验检测LPS-DC及IFN-DC抗肿瘤活性的差异.结果①LPS及IFN-γ可不同程度的上调DC表面CD86、CD80、CD83及CD1a的表达,以LPS刺激组明显.②IFN-γ和LPS可分别增强DC对HL60及Daudi的杀伤活性,在效靶比为20∶1及10∶1时杀伤率与未加刺激因子对照组(medium-DC)相比差异有显著意义(P＜0.05).相反,IFN-γ-DC对Daudi、LPS-DC对HL-60无明显杀伤活性,但两者对Jurkat均具杀伤作用.结论 LPS及IFN-γ激活的DC对肿瘤细胞的杀伤活性具有相对肿瘤特异性.
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