You Position: Home > Paper
Influence of Bushen Huoxue decoction(补肾活血汤)on β-catenin, MMP-7 of synoviocytes in rats with knee osteoarthritis
( views:382, downloads:145 )
- Author:
- No author available
- Journal Title:
- China Journal of Orthopaedics and Traumatology
- Issue:
- 9
- DOI:
- 10.3969/j.issn.1003-0034.2012.09.014
- Key Word:
- 骨关节炎,膝;补肾活血方;基质金属蛋白酶7;动物实验
Abstract： 目的:观察Wnt经典信号通路中β-catenin在大鼠膝骨关节炎滑膜细胞胞核中的表达,并观察补肾活血方对大鼠膝骨关节炎(OA)膝关节滑膜细胞胞核中β-catenin和滑膜细胞MMP-7的调控作用.方法:采用Hulth法建立大鼠膝关节骨性关节炎模型,胶原酶消化法原代培养正常滑膜细胞与OA滑膜细胞.将所培养滑膜细胞分为正常组、OA模型组和补肾活血方组,用补肾活血方含药血清作用OA滑膜细胞48 h后,采用Western Blot法检测滑膜细胞中MMP-7、胞核β3-catenin的蛋白表达变化；采用酶联免疫吸附法(ELISA)检测滑膜细胞上清液中MMP-7表达变化.结果:OA滑膜细胞培养成功,Western Blot结果显示OA滑膜细胞中MMP-7、胞核β-catenin表达均明显高于正常滑膜细胞,补肾活血方对OA滑膜细胞中MMP-7、胞核β-catenin表达有明显下调作用；ELISA结果显示OA滑膜细胞上清液中MMP-7表达明显高于正常滑膜细胞上清液,补肾活血方对其有明显下调作用.结论:①β-catenin在OA滑膜细胞胞核中表达升高,表明Wnt经典信号通路在大鼠膝骨关节炎滑膜中是激活的.②MMP-7在OA滑膜细胞和上清液中表达升高,印证了MMP-7是Wnt/β catenin信号通路下游基因.③滑膜细胞Wnt/β-catenin信号通路的活化、MMP-7的表达升高可能是导致关节软骨降解,促进骨性关节炎形成的机制之一.④补肾活血方对Wnt/β-catenin信号通路的调控作用是其抑制软骨降解,促进软骨修复,治疗骨性关节炎的可能机制之一.
- [1]陈智能,杨连梓.滑膜性病变与骨性关节炎[J].2003,(4).
- [2]蒋建平,杨铁城,侯凡凡,等.人类关节滑膜A型和B型细胞的分离和体外培养[J].2001,(1).
- [3]阚卫兵,袁琴,宋鹏飞,等.自拟补肾活血方对家兔膝关节骨性关节炎模型滑膜组织MMPs的影响[J].2011,(2).
- [4]王朴.生地黄的现代药理研究与临床应用[J].2008,(8).
- [5]刘芳.当归的心脑血管药理作用研究进展[J].2010,(6).
- [6]汤继文,刘新宇.骨关节病的诊治[J].2000,(15).
- [7]吕大鹏,白伦浩,孔冉冉,等.MMP-7、MMP-9及其抑制剂TIMP-3在骨性关节炎患者膝关节滑膜中的表达和意义[J].2009,(4).
- [8]刘屏,王东晓,陈桂芸,等.鸡血藤单体化合物对造血祖细胞增殖的调控作用研究[J].2007,(6).
- [9]Wang YJ,Chen M,O'Keefe RJ,etc.Inhibition of beta-catenin signaling in articular chondrocytes results in articular cartilage destruction.[J].2008,58(7).
- [10]Sutton S,Mobasheri A,Clutterbuck A,etc.The contribution of the synovium, synovial derived inflammatory cytokines and neuropeptides to the pathogenesis of osteoarthritis[J].2009,179(1).
- [11]李献平,刘世昌.四大怀药对家蚕寿命及生长发育的影响[J].1990,(9).
- [12]Cornelia, Am?linei,Irina Draga, C?runtu,Simona Eliza, Giu?c,etc.Matrix metalloproteinases involvement in pathologic conditions.[J].2010,51(2).
- [13]戴伟礼,李根池.小鼠甲醛致痛模型筛选中药牛膝的镇痛作用[J].1989,011 (10).
- [14]Wu Q,Zhu M,Tang D.Activation of beta-catenin signaling in articular chondrocytes leads to osteoarthritis-like phenotype in adult beta-catenin conditional activation mice.[J].2009,24(1).
- [15]Tamamura Y,Otani T,Kitagaki J,etc.Developmental regulation of Wnt/beta-catenin signals is required for growth plate assembly, cartilage integrity, and endochondral ossification[J].2005,280(19).
- [16]Goldring MB,Goldring SR.Osteoarthritis.[J].2007,213(3).