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Cardiac protective effect of granulocyte colony-stimulating factor combined with ischemic postconditioning on acute myocardial infarction in rabbits
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PATHOPHYSIOLOGY
- Issue:
- 11
- DOI:
- 10.3969/j.issn.1000-4718.2012.11.003
- Key Word:
- 粒细胞集落刺激因子;缺血后适应;心肌梗死;再灌注损伤
Abstract： 目的:探讨粒细胞集落刺激因子(G-CSF)联合缺血后适应(IP)对实验兔急性心肌梗死的心脏保护作用.方法:将兔结扎左室支(LVA)后分为4组:缺血再灌注模型组(IR组,松结后直接再灌注)、G-CSF组(松结并于术后12 h开始使用G-CSF皮下注射)、IP组(予以IP行改良再灌注)和G-CSF + IP组(联合应用IP及G-CSF干预).观察各组术中心电图ST段变化,检测手术前后血常规和心肌钙蛋白I(cTnI),术后4周行超声心动图检查,测定各组梗死心肌的面积、组织病理结构和细胞凋亡.结果:IP组术中ST段回落快于模型组;G-CSF处理后,实验动物血常规中白细胞明显增高;IP组、G-CSF组及联合治疗组术后7 d的cTnI明显低于模型组; 术后4周超声心动图结果显示,IP组、G-CSF组及联合治疗组指标好于模型组,联合治疗组最佳; IP组、G-CSF组及联合治疗组梗死心肌面积均明显低于模型组,梗死周边细胞凋亡程度低、血管密度高,联合治疗组最好.结论:G-CSF联合IP可有效减轻心肌梗死后急性期的再灌注损伤,有利于恢复期的心脏修复.
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