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Regulation of triptolide on histone demethylase in multiple myeloma U266 cells
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- Chinese Traditional and Herbal Drugs
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- 10
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- 雷公藤内酯醇;多发性骨髓瘤U266细胞;细胞凋亡;细胞增殖;组蛋白去甲基化酶;表观遗传
Abstract： 目的 研究雷公藤内酯醇对多发性骨髓瘤U266细胞组蛋白去甲基化酶的影响,探讨其表观遗传调控作用.方法 采用MTT法检测细胞增殖活性:Annexin V-FITC/PI双标法流式细胞术检测细胞凋亡;RT-PCR法检测U266细胞组蛋白赖氨酸去甲基化酶1 (LSDI) mRNA、组蛋白去甲基化酶(JMJD2B)mRNA表达的变化;激光共聚焦显微技术观察LSDI亚细胞定位情况及蛋白量的变化;Western blotting法检测在雷公藤内酯醇干预下LSD1和JMJD2B蛋白表达的变化.结果 雷公藤内酯醇以剂量、时间相关方式抑制U266细胞增殖,与细胞培养24 h的IC50为153.2 nmol/L;以剂量相关方式诱导U266细胞凋亡,雷公藤内酯醇80 nmol/L作用U266细胞24 h后,细胞出现典型的凋亡形态学改变,总凋亡率达32.9％.;以剂量相关方式抑制JMJD2B蛋白的表达,同时促进LSD1蛋白表达.结论 雷公藤内酯醇在抑制U266细胞增殖、诱导其凋亡的同时,明显改变LSD1、JMJD2B的表达,其诱导U266细胞凋亡和抗肿瘤效应可能与其调节LSD1、JMJD2B表达有关.
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