You Position: Home > Paper
Optimization on preparation technology of ardipusilloside-Ⅰ polylactic acid microspheres by orthogonal design
( views:193, downloads:0 )
- Author:
- No author available
- Journal Title:
- Chinese Traditional and Herbal Drugs
- Issue:
- 10
- DOI:
- No doi available
- Key Word:
- 九节龙皂苷Ⅰ;聚乳酸;微球;HPLC-ELSD;正交设计
Abstract： 目的 筛选溶剂蒸发法制备九节龙皂苷Ⅰ聚乳酸微球(ADS-Ⅰ-PLA-MS)最佳工艺.方法 采用HPLC-ELSD测定方法,以包封率和载药量为评价指标,W/O/W溶剂蒸发法制备微球;通过单因素和正交试验设计,考察内水相九节龙皂苷Ⅰ (ADS-Ⅰ)甲醇溶液的质量浓度、ADS-Ⅰ甲醇溶液与聚乳酸(PLA)二氯甲烷溶液体积比、PLA二氯甲烷溶液质量浓度和聚乙烯醇(PVA)体积等因素对ADS-Ⅰ-PLA-MS包封率及载药量的影响.结果 溶剂蒸发法制备ADS-Ⅰ-PLA-MS的最佳工艺条件为ADS-Ⅰ甲醇溶液质量浓度为8 mg/mL、ADS-Ⅰ甲醇溶液与PLA二氯甲烷溶液体积比为1∶13、PLA二氯甲烷溶液质量浓度为90 mg/mL、PVA体积为500 mL.结论 优选出的ADS-Ⅰ-PLA-MS制备工艺合理可行.
- [1]谭载友,廖清江,肖罗宾,等.两种乳化技术制备的爱普列特微球的特性研究[J].2003,(4).
- [2]Gu,Y.,Zhou,S.-Y.,Zhang,B.-L.,etc.Metabolism and pharmacokinetic study of ardipusilloside i in rats[J].2012,78(6).
- [3]罗宇燕,张永明,吴传斌.一种聚乳酸聚乙醇酸缓释微球的制备工艺[J].2011,(11).
- [4]玉石,李娟,张晓楠.九节龙皂苷诱导胶质瘤SHG-44细胞凋亡的实验观察[J].2009,(14).
- [5]林雅铃,张安强,王炼石.磷酸川芎嗪星型聚乳酸缓释微球的制备与体外释放研究[J].2010,(10).
- [6]HaiFeng Tang,Xiang Zhang,Hai Ning Zhen,etc.Ardipusilloside I induces apoptosis in human glioblastoma cells through a caspase-8-independent FasL/Fas-signaling pathway[J].2009,27(2).
- [7]Ong BY,Ranganath SH,Lee LY,etc.Paclitaxel delivery from PLGA foams for controlled release in post-surgical chemotherapy against glioblastoma multiforme.[J].2009,30(18).
- [8]An TK,Lee CO,Lee HB,etc.BCNU-loaded poly(D, L-lactide-co-glycolide) wafer and antitumor activity against XF-498 human CNS tumor cells in vitro.[J].2003,251(1).
- [9]麻育源,兰青.人脑胶质瘤间质内化疗研究进展[J].2004,(3).