Abstract： Objective To study the chemical constituents of Oxytropis kansuensis systematically. Methods Silica gel column chromatographic method was used to isolate and purify the constituents from the extract of O. kansuensis. The structures of the compounds were elucidated by various spectroscopic methods including NMR spectrometer and high resolution mass spectrometry, and the known compounds were identified on the basis of comparing their NMR data with those of corresponding compounds in the lit-erature. Results Thirteen compounds were isolated from O. kansuensis and elucidated as rhamnocitrin (1), rhamnocitrin-3-O-β-D-galactopyranoside (2), rhamnocitrin-3-O-β-D-galactopyranoside-4 -O-β-D-glu-curonospyranoside (3), (±)-10-methoxymedicarpin (4), 7β-hydroxysitosterol (5), 7α-hydroxysitosterol (6), 5, 11-stigmastadien-3β-ol (7), β-sitosterol (8), β-daucosterin (9), soyasapogenol B (10), 3-O-[-β-D-glucopyranosyl (1-2)-β-D-glucopyranosyl-] soyasapogenol B (11), 3-O-[α-L-rhamnopyranosyl (1-2)-β-D-glucopyranosyl (1-2)-β-D-glucopyranosyl] soyasapogenol B (12), DL-3-O-methyl-inositol (13). Conclu-sion The compounds are all isolated from this plant for the first time.