Abstract: Objective To investigate the chemical constituents in the whole plant of Hedyotis coryrnbo-sa. Methods The compounds were isolated by column chromatography, pre-TLC, and reerystallization. Their structures were elucidated by spectroscopic methods. Results Thirteen compounds were isolated and identified as (+)-lyoniresinol-3a-O-β-D-glucopyranoside (Ⅰ), quercetin (Ⅱ), esculetin (Ⅲ), scopo-letin (Ⅳ), hedyotiscone A (Ⅴ), p-hydroxybenzoic acid (Ⅵ), protocatechuic acid (Ⅶ), vanillic acid (Ⅷ), syringic acid (Ⅸ), (+)-vomifoliol (Ⅹ), (-)-dihydrovomifoliol (Ⅺ), S-(+)-dehydrovomifoliol(Ⅻ), and alizarin 1-methyl ether (ⅩⅢ ), respectively. Conclusion Compounds Ⅰ- are isolated from this plant for the first time.