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LC-MS Analysis of Buzhongyiqi Tang and its regulating effect on immune-depressed mice
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- Author:
- No author available
- Journal Title:
- CHINESE TRADITIONAL AND HERBAL DRUGS
- Issue:
- 8
- DOI:
- 10.3321/j.issn:0253-2670.2006.08.005
- Key Word:
- 补中益气汤;LC-MS;免疫;环磷酰胺
Abstract： 目的 研究补中益气汤的主要化学成分及其对环磷酰胺免疫抑制小鼠的调节作用.方法 采用LC-MS方法对补中益气汤进行了液相和质谱分析,通过MTT法测定补中益气汤对环磷酰胺免疫抑制小鼠的T、B淋巴细胞增殖和NK细胞活性的影响.结果 补中益气汤中主要化学成分是多糖、黄酮类和皂苷类化合物,能够显著提高环磷酰胺免疫抑制小鼠的T、B淋巴细胞增殖能力和NK细胞活性.结论 LC-MS方法可以发展成为复方有效成分研究中一个有效手段,用来阐明复方药理作用的物质基础.
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