Abstract: Object To study the insecticidal activity constituents from the twigs of traditional Chinese folk medicine "Mizilan" or "Shu-Lan" (Aglaia odorata Lour. ). Methods Seperation and purification under bioassay-directed were performed on silica gel CC, Sephadex LH-20 CC and preparative TLC. Their structures were established on the basis of physicochemical and spectral analysis. Results Seven compounds were isolated and identified as rocaglamide ( Ⅰ ), desmethyl rocaglamide ( Ⅱ ), 8-methoxymarikarin (Ⅲ), 7-hydroxy-6-methoxy-coumarin (Ⅳ), 3′-hydroxy-methylrocaglate (Ⅴ), 3′-hydroxyrocaglamide (Ⅵ), marikarin (Ⅶ), respectively. Conclusion Compound Ⅳ is isolated from the plant of Aglaia Lour. for the first time and compounds Ⅲ and Ⅶ are isolated from A. odorata for the first time.