Abstract: Object To study the chemical constituents in lichen plant of Cladonia stellaris. Methods C. Stellaris was extracted with petroleum ether,separated and purified by column chromatography and preparative TLC on silica gel. All the compounds were identified on the basis of spectral analysis. Results Six compounds were obtained. Their structures were characterized as usnic acid(Ⅰ),evinic acid (Ⅱ),perlatolic acid(Ⅲ),olivetoric acid (Ⅳ),2-hydroxyl-4-methoxyl-6-pentyl benzoic acid(Ⅴ) and 2,4-dihydroxyl benzoic acid (Ⅵ),respectively. Conclusion Compounds Ⅱ and Ⅳ were isolated from C. Stellaris for the first time.