Biotransformation of artemisinin by fermentation of Rhizopus chinensis and Cunninghamella elegans
- Author:
- No author available
- Journal Title:
- CHINESE TRADITIONAL AND HERBAL DRUGS
- Issue:
- 10
- DOI:
- 10.3321/j.issn:0253-2670.2002.10.002
- Key Word:
- 生物转化;青蒿素;华根霉;雅致小克银汉霉;biotransformation;artemisinin;Rhizopus chinensis Saito;Cunninghamella elegans Lendn
Abstract： Object To study the microbial transformation of antimalarial drug artemisinin ( I ).Methods The enzymes secreted by fermentation of Rhizopus chinensis Saito CICC 3043 and Cunninghamel-la elegans Lendn. AS 3. 1207 in the potato medium were employed to transform artemisinin. ResultsThree products were obtained, among which deoxyartemisinin ( Ⅱ ) was also found in the controls withoutmicroorganisms. The other two were identified as 3α-hydroxydeoxyartemisinin (Ⅲ) and 9β-hydrox-yartemisinin (Ⅳ). Compound (Ⅳ) was identified as a new one. Conclusion Artemisinin could be trans-formed by the two title strains of microorganisms. It is easy to release one oxygen atom by breaking of perexide bridge in potato medium and to form deoxyartemisinin, thus making it lose its antimalarial activi-ty. The iron (Fe) in the potato medium may have the above function.
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