Abstract: Object To investigate the chemical constituents from the stem of Pueraria peduncularis (Grah. ex Benth. ) Benth. Methods The constituents were isolated on silica gel column chromatography.Their structures were elucidated by chemical and spectroscopic evidence. Results Ten compounds were comfirmed as 3-O- [β-D-glucopyranosyl- ( 1 → 3 )-β-D- 6-O-methyl-glucuronopyranosyl ]- 3β3, 1 Sa, 2 3-trihydroxy-olean-12-en-16-one (Ⅰ), daidzein (Ⅱ), genistein ( Ⅲ ), daidzin ( Ⅳ ), genistin ( V ), 3β3, 15α-dihydroxy-olean-12-en-16-one (VⅥ), lupeol (Ⅶ), betulinic acid (Ⅷ), α-spinasterol-glucopyranoside (ⅨX)and α-spinasterol ( X ). Conclusion Compound I exhibited antimicrobial activity against Aspergillus niger.Compounds Ⅱ~ X were first isolated from this plant.