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Purification of fibrinolytic enzyme from Perinereis aibuhitensis
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- Author:
- No author available
- Journal Title:
- CHINESE TRADITIONAL AND HERBAL DRUGS
- Issue:
- 8
- DOI:
- 10.3321/j.issn:0253-2670.2001.08.001
- Key Word:
- 双齿围沙蚕;纤溶酶;分离纯化
Abstract： 目的从双齿围沙蚕体内提取并纯化一种新的纤溶酶.方法应用硫酸铵沉淀、透析、凝胶柱层析(Sephadex G-100)以及电泳等生化手段对沙蚕纤溶酶进行分离纯化.用平板法测定它降解纤维蛋白的活性.结果得到纯的沙蚕纤溶酶,测出其等电点为4.5左右,由两条肽链组成,其相对分子量分别为33 000 u和14 400 u.结论该酶为首次从沙蚕中发现的一种新的纤溶酶.
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