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Advance in Research on Bacterial Aromatic Extradiol Dioxygenase

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Author:: No author available

Journal Title:: Chinese Journal of Applied & Environmental Biology

Issue:: 5

DOI:: 10.3724/SP.J.1145.2012.00873

Key Word:: 外二醇双加氧酶;分类;催化机制;生物降解;生物合成;生物技术

Abstract：外二醇双加氧酶(EDOs)是一种多功能细菌芳烃开环氧化酶,在环境保护、化工合成及生物技术等领域中有着巨大的应用潜力.本文综述了EDOs自开发以来的研究成果,包括分类学研究,酶的催化机制,在生物降解、生物合成、生物技术中的应用及其开发改造新技术.EDOs属于3个进化关系相互独立的酶家族,它们利用活性位点金属离子Fe/Mn(Ⅱ)与底物和氧气结合,通过形成一种烷基过氧化中间产物,使芳香化合物开环断裂.利用这一催化机制,EDOs可以广泛地降解多种环境污染物,同时,某些EDOs还能够参与生物活性物质的合成,并且在生物传感器等生物技术中也有着广泛的应用.近年来,结合宏基因组、杂交酶等技术手段,研究人员开发改造出更多的EDOs资源,旨在为EDOs的深入研究提供更全面的信息.

Reference

[1]吕鹏,史建国,冯东,等.邻苯二酚双加氧酶传感器的研究[J].2005,(4).
[2]任河山,王颖,赵化冰,等.酚降解菌株的分离、鉴定和在含酚废水生物处理中的应用[J].2008,(2).
[3]S, Harayama,M, Rekik.Bacterial aromatic ring-cleavage enzymes are classified into two different gene families.[J].1989,264(26).
[4]Akiko Okuta,Kouhei Ohnishi,Shigeaki Harayama.PCR isolation of catechol 2,3-dioxygenase gene fragments from environmental samples and their assembly into functional genes[J].1998,212(2).
[5]刘志培,杨惠芳,周培谨.微生物降解苯胺的特性及其降解代谢途径[J].1999,(Z1).
[6]Eltis LD,Vaillancourt FH,Bolin JT.The ins and outs of ring-cleaving dioxygenases.[J].2006,41(4).
[7]S, Han,L D, Eltis,K N, Timmis,etc.Crystal structure of the biphenyl-cleaving extradiol dioxygenase from a PCB-degrading pseudomonad.[J].1995,270.
[8]K N, Timmis,R J, Steffan,R, Unterman.Designing microorganisms for the treatment of toxic wastes.[J].1994,48.
[9]Zhang, Q.,Zhang, X.,Zhou, J.,etc.Extradiol dioxygenase-SiO_2 sol-gel modified electrode for catechol and its derivatives detection[J].2011,26(11).
[10]Pierre-Alain Girod,Jean-Pierre Zryd.Biogenesis of betalains: Purification and partial characterization of dopa 4,5-dioxygenase from Amanita muscaria[J].1991,30(1).
[11]Hikaru Suenaga,Shiori Mizuta,Kentaro Miyazaki.Themolecular basis foradaptive evolution in novel extradiol dioxygenases retrieved fromthemetagenome[J].2009,69(3).
[12]Mendel S,Bugg TDH,Arndt A.Acid-base catalysis in the extradiol catechol dioxygenase reaction mechanism: site-directed mutagenesis of his-115 and his-179 in Escherichia coli 2,3-dihydroxyphenylpropionate 1,2-dioxygenase (MhpB)[J].2004,43(42).
[13]Kovaleva EG,Lipscomb JD.Versatility of biological non-heme Fe(II) centers in oxygen activation reactions[J].2008,4(3).
[14]Jonathan Sanvoisin,G. John Langley,Timothy D. H. Bugg.Mechanism of Extradiol Catechol Dioxygenases: Evidence for a Lactone Intermediate in the 2,3-Dihydroxyphenylpropionate 1,2-Dioxygenase Reaction[J].1995,117(29).
[15]Brennerova, MV,Josefiova, J,Brenner, V,etc.Metagenomics reveals diversity and abundance of meta-cleavage pathways in microbial communities from soil highly contaminated with jet fuel under air-sparging bioremediation[J].2009,11(9).
[16]R I, Amann,W, Ludwig,K H, Schleifer.Phylogenetic identification and in situ detection of individual microbial cells without cultivation.[J].1995,59(1).
[17]Fernand Terradas,Hugo Wyler.The secodopas, natural pigments in Hygrocybe conica and Amanita muscaria[J].1991,30(10).
[18]L D, Eltis,J T, Bolin.Evolutionary relationships among extradiol dioxygenases.[J].1996,178(20).
[19]Lipscomb JD.Mechanism of extradiol aromatic ring-cleaving dioxygenases[J].2008,18(6).
[20]Kovaleva EG,Lipscomb JD.Crystal structures of Fe2+ dioxygenase superoxo, alkylperoxo, and bound product intermediates.[J].2007,316(5823).
[21]Christinet L,Burdet FRX,Zaiko M,etc.Characterization and functional identification of a novel plant 4,5-extradiol dioxygenase involved in betalain pigment biosynthesis in Portulaca grandiflora[J].2004,134(1).
[22]Narita H.,Sugiyama K.,Kimbara K.,etc.Three-Dimensional Structures of Free Form and Two Substrate Complexes of an Extradiol Ring-Cleavage Type Dioxygenase, the Bphc Enzyme from Pseudomonas Sp Strain Kks102[J].1996,255(5).
[23]Dunwell JM,Culham A,Carter CE,etc.Evolution of functional diversity in the cupin superfamily.[J].2001,26(12).
[24]Fortin PD,MacPherson I,Neau DB,etc.Directed evolution of a ring-cleaving dioxygenase for polychlorinated biphenyl degradation[J].2005,280(51).