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PLFA Fingerprint Method for Assessing Microbial Communities of Strong Aromatic Liquor Pit Mud
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Applied & Environmental Biology
- Issue:
- 5
- DOI:
- 10.3724/SP.J.1145.2012.00831
- Key Word:
- 磷脂脂肪酸;窖泥;微生物群落;指纹图谱;主成分分析;浓香型白酒
Abstract： 针对白酒发酵过程中窖泥微生物群落的特殊性,以不同类型泥土和常见酿酒微生物为研究对象,构建了研究浓香型白酒窖泥微生物群落的磷脂脂肪酸(PLFA)指纹图谱法.结果表明:不同类型样品PLFA指纹图谱的显著差异(P＜0.05)体现为样品PLFA的组成及含量受其生理及环境条件的影响,发生了显著的变化(花园和路旁泥土的真菌与细菌特征PLFA的比值分别为0.30和0.66,均小于1,而窖泥为1.17)；5种常见微生物纯培养物加入窖泥后使得与其类型相对应的特征PLFA含量变化显著(添加SICC1.13的窖泥中a14:0增加了1.44倍,CICC20633使a16:0增加了9.92倍,SICC3398使18:2ω6,9增加了82.58倍,SICC31482使18:2ω6,9增加了90.33倍,添加DH5α的窖泥中检出了cy17:0)(P＜0.05)；应用主成分分析( PCA)揭示了窖泥中加入不同微生物菌株后其特征PLFA的组成及含量的变化规律.研究佐证了PLFA指纹图谱法是一种表征酿酒过程微生物群落指纹图谱快捷的生化免培养法.
- [1]王曙光,侯彦林.磷脂脂肪酸方法在土壤微生物分析中的应用[J].2004,(1).
- [2]喻曼,曾光明,陈耀宁,等.PLFA法研究稻草固态发酵中的微生物群落结构变化[J].2007,(11).
- [3]潘响亮,邓伟,张道勇.磷脂脂肪酸在地下水微生物生态学中的应用及存在的若干问题[J].2003,(6).
- [4]许文涛,郭星,罗云波,等.微生物菌群多样性分析方法的研究进展[J].2009,(7).
- [5]任瑞霞,张颖,李慧,等.石油污染土壤水改旱田后污染物组份及微生物群落结构变化[J].2007,(5).
- [6]张文学,乔宗伟,胡承,等.PCR技术对浓香型白酒糟醅细菌菌群的解析[J].2005,(5).
- [7]张文学,乔宗伟,胡承,等.浓香型白酒糟醅中真菌菌群的多样性分析[J].2006,(5).
- [8]DC White,JO StairDB Ringelberg.Quantitative comparisons ofin situ microbial biodiversity by signature biomarker analysis[J].1996,17(3-4).
- [9]L. Zelles.Fatty acid patterns of phospholipids and lipopolysaccharides in the characterisation of microbial communities in soil: a review[J].1999,29(2).
- [10]郑慧,祁士华,王志勇,等.高位池养虾对土壤微生物脂肪酸和土壤酶活及其表征的土壤质量的影响[J].2011,(1).
- [11]D.C.White,W.M.Davis,J.S.Nickels,etc.Determination of the sedimentary microbial biomass by extractible lipid phosphate[J].1979,40(1).
- [12]T L, Kieft,D B, Ringelberg,D C, White.Changes in Ester-Linked Phospholipid Fatty Acid Profiles of Subsurface Bacteria during Starvation and Desiccation in a Porous Medium.[J].1994,60.
- [13]王蓓,孙庚,罗鹏,等.模拟升温和放牧对高寒草甸土壤微生物群落的影响[J].2011,(2).
- [14]冯广达,邓名荣,郭俊,等.利用PCR-DGGE溯源典型农村塘坝饮用水中的污染[J].2011,(3).
- [15]Rinklebe J,Langer U.Microbial diversity in three floodplain soils at the Elbe River (Germany).[J].2006,38(8).
- [16]L. Zelles,Q.Y. Bai.Fractionation of fatty acids derived from soil lipids by solid phase extraction and their quantitative analysis by GC-MS[J].1993,25(4).
- [17]A, Frosteg?rd,A, Tunlid,E, B??th.Phospholipid Fatty Acid composition, biomass, and activity of microbial communities from two soil types experimentally exposed to different heavy metals.[J].1993,59(11).
- [18]Rillig MC,Gannon JE,Ramsey PW,etc.Choice of methods for soil microbial community analysis: PLFA maximizes power compared to CLPP and PCR-based approaches[J].2006,50(3).
- [19]Baath, Erland .,Tunlid, Anders,Frostegard, Asa.Use and misuse of PLFA measurements in soils[J].2011,43(8).
- [20]L. Zelles.Identification of single cultured micro-organisms based on their whole-community fatty acid profiles, using an extended extraction procedure[J].1999,39(4).