You Position: Home > Paper
Microencapsulation of Lactic Acid Bacteria with Improved Performance by Spray-congealing
( views:69, downloads:0 )
- Author:
- No author available
- Journal Title:
- Chinese Journal of Applied & Environmental Biology
- Issue:
- 5
- DOI:
- 10.3724/SP.J.1145.2012.00824
- Key Word:
- 微胶囊;乳酸菌;喷霉冷凝法;变性淀粉;胃肠道存活率
Abstract： 活菌制剂进入动物消化道后由于经受胃酸、胆盐以及消化酶等的作用而造成活性丧失及肠道定殖困难是目前乳杆菌制剂应用的瓶颈问题.本研究通过喷雾冷凝法制备干酪乳杆菌微胶囊以增强乳杆菌在人体的益生功能.选用不同浓度的海藻酸钠溶液与氯化钙溶液作为壁材,考察喷雾冷凝法制备乳杆菌微胶囊的包埋率、胃肠道释放等情况.通过比较微胶囊的包埋率和粒径分布的效果后,选择海藻酸钠浓度为2％、氯化钙浓度为3％的芯壁材(包埋率最高达95.80％)制备干酪乳杆菌微胶囊.初始活菌数为109 cfu/mL的乳酸菌微胶囊经模拟人体胃液处理3h后,仅有24.17％的活菌溢出,证明微胶囊处理后的乳杆菌能够抵抗较低pH值及胃蛋白酶的作用.之后将微胶囊置人模拟人体肠液中,60 min后微胶囊释放率达到84.22％,表明上述方法制备的乳杆菌微胶囊能够在肠道中释放定殖并发挥其益生作用.进一步研究表明,添加变性淀粉作为益生元,能够增强乳酸菌微胶囊的耐酸性能,并且在肠液中释放后活菌出现继续增殖的现象.上述研究结果为选择高效保护剂应用喷雾冷凝法制备乳酸菌微胶囊奠定了研究基础.
- [1]刘晓庚,谢亚桐.微胶囊制备方法的比较[J].2005,(1).
- [2]郭佳,陈琳,张柏林.膳食纤维微胶囊化包埋益生菌的研究[J].2008,(5).
- [3]庄海宁,张燕萍,金征宇.第三类抗消化淀粉（RS3）在益生菌微胶囊方面的应用进展[J].2008,(6).
- [4]Mooney DJ,Madlambayan G,Rowley JA.Alginate hydrogels as synthetic extracellular matrix materials.[J].1999,20(1).
- [5]P, Marteau,M C, Boutron-Ruault.Nutritional advantages of probiotics and prebiotics.[J].2002,87 Suppl 2.
- [6]Zhao Ruixiang,Sun Junliang,Torley Peter,etc.Measurement of particle diameter of Lactobacillus acidophilus microcapsule by spray drying and analysis on its microstructure[J].2008,24(8).
- [7]Junyaprasert VB,Manwiwattanakul G.Release profile comparison and stability of diltiazem-resin microcapsules in sustained release suspensions.[J].2008,352(1/2).
- [8]Gbassi Gildas Komenan,Vandamme Thierry,Ennahar Said.Microencapsulation of Lactobacillus plantarum spp in an alginate matrix coated with whey proteins[J].2009,129(1).
- [9]Kleessen, Brigitta,Stoof, Gisela.Feeding resistant starch affects fecal and cecal microflora and short-chain fatty acids in rats.[J].1997,75(9).
- [10]Maschke A,Eyrich D,Kiermaier J,etc.Development of a spray congealing process for the preparation of insulin-loaded lipid microparticles and characterization thereof.[J].2007,65(2).
- [11]Topping DL,Fukushima M,Bird AR.Resistant starch as a prebiotic and synbiotic: state of the art[J].2003,62(1).
- [12]O, Smidsr?d,G, Skj?k-Braek.Alginate as immobilization matrix for cells.[J].1990,8.
- [13]Chavarri M,Maranon I,Ares R,etc.Microencapsulation of a probiotic and prebiotic in alginate-chitosan capsules improves survival in simulated gastro-intestinal conditions.[J].2010,142(1/2).
- [14]Ana Blandino,Manuel Macías,Domingo Cantero.Formation of calcium alginate gel capsules: Influence of sodium alginate and CaCl2 concentration on gelation kinetics[J].1999,88(6).
- [15]Kaila Kailasapathy.Microencapsulation of Probiotic Bacteria: Technology and Potential Applications[J].2002,3(2).
- [16]Gibbs BF.,Kermasha S.,Alli I.,etc.Encapsulation in the food industry: a review[J].1999,50(3).
- [17]Katan MB,de Roos-NM.Effects of probiotic bacteria on diarrhea, lipid metabolism, and carcinogenesis: a review of papers published between 1988 and 1998.[J].2000,71(2).
- [18]A A, Zuppa,I, Savarese,A, Scorrano,etc.[Prebiotics and probiotics in infant nutrition].[J].1900,29.
- [19]Stanton C,Fitzgerald GF,Desmond C,etc.Overcoming the technological hurdles in the development of probiotic foods[J].2005,98(6).