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Improvement of Two-dimensional Electrophoresis of Rice (Oryza sativa L.) Proteomics
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Applied & Environmental Biology
- Issue:
- 5
- DOI:
- 10.3724/SP.J.1145.2012.00819
- Key Word:
- 水稻;双向电泳;蛋白质组学;5％冰乙酸;考马斯亮蓝R-250
Abstract： 为获得高分辨率、高灵敏度的2-DE图谱,以3叶1心期的水稻幼苗叶片为研究材料,对蛋白样本的制备、上样量及考马斯亮蓝R-250染色等关键步骤进行探讨,优化实验条件.结果表明,脱色后凝胶经5％冰乙酸浸泡后得到的2-DE图谱蛋白质点明显增多,图谱背景清晰.同时,不同公司考染试剂CBB R-250染色灵敏度存在较大的差别,应予以尝试.同其它方法相比,本方法简便、稳定,灵敏度高,可操作性强,对水稻蛋白质组学研究具有一定的指导意义.
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