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Isolation, Identification, Culture Conditions, and Enzyme Production of a Wild Mushroom Lepista sp.
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Applied & Environmental Biology
- Issue:
- 5
- DOI:
- 10.3724/SP.J.1145.2012.00804
- Key Word:
- 紫丁香蘑;ITS鉴定;培养条件;漆酶;Cu2+
Abstract： 为充分开发利用野生食用菌资源,从采集自北京怀柔的野生香蘑子实体中分离获得纯培养,编号为Lepista sp.GSM-11,结合形态学特征与ITS序列分析鉴定其分类学地位,并进一步研究其菌丝生长的最适碳源、氮源、C/N比、生长因子、最适温度和最适pH值,并测定了菌体液体发酵产漆酶、蛋白酶、植酸酶、核糖核酸酶和凝集素的特性,同时还研究了Cu2+对漆酶产量的影响.结果表明,该菌株与GenBank中报道的采集自中国辽宁、美国马里兰州和日本宫城的紫丁香蘑(Lepista nuda)同源性最高,分别为100％、99.98％和99.93％,确定该野生香蘑为紫丁香蘑(ITS序列GenBank 登录号JQ519375)；菌丝体生长的最适碳源为蔗糖,最适氮源为酵母浸膏,最适C/N比为20/1,最适生长因子为维生素C,最适温度为24℃,最适pH为7.0; GSM-11菌株菌丝体和发酵液中均具有漆酶活性,但缺乏其他3种酶和凝集素活性.1.0～2.0 mmol/L的Cu2+对漆酶分泌有显著的促进作用,其活性达到对照组的149.2％～196.4％.
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