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Glycerol Synthesis Attenuation of Candida glycerinogenes in Fed-batch Fermentation
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Applied & Environmental Biology
- Issue:
- 5
- DOI:
- 10.3724/SP.J.1145.2012.00791
- Key Word:
- 产甘油假丝酵母;补料发酵;甘油合成衰减;代谢流;关键酶
Abstract： 为揭示产甘油假丝酵母Candida glycerinogenes补料发酵后甘油合成衰减机理,以指导甘油合成及菌株改造,对补料发酵后甘油的生成情况、葡萄糖的消耗情况、细胞内的代谢流量变化以及代谢途径关键酶活力变化进行了研究.结果表明,补料发酵后葡萄糖的代谢流量分布发生变化,流向HMP途径和甘油合成途径的流量分别降低48％和33％,更多的碳流通过EMP途径形成副产物；胞浆3-磷酸甘油脱氢酶(ctGPD)和6-磷酸葡萄糖脱氢酶(G6PDH)的活力下降,而丙酮酸激酶(PYK)活力上升.补料发酵后甘油合成关键酶ctGPD酶活力低下,流向甘油合成途径的碳流减小,还原力供给受影响,这些因素共同作用引起了补料发酵后甘油合成的衰减.
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