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Optimization of Fermentation Conditions of Marine Algicidal Bacterium Microbulbifer sp.BS03 by Uniform Design
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Applied & Environmental Biology
- Issue:
- 5
- DOI:
- 10.3724/SP.J.1145.2012.00761
- Key Word:
- 杀藻细菌;单因素试验;均匀设计;塔玛亚历山大藻;发酵条件
Abstract： 采用均匀设计法设计和二次多项式逐步回归分析,对一株高效杀塔玛亚历山大藻微泡菌BS03(Microbulbifer sp.)产杀藻活性物质的发酵培养条件进行优化.通过单因素实验筛选出碳源、氮源、pH、培养时间和接种量为显著影响因子,并对5个显著影响因子采用U15( 155)水平对培养基进行优化.结果表明BS03最适发酵培养条件为:蔗糖8 g/L,蛋白胨10.50 g/L,初始pH值7.5,培养时间32 h,接种量3.00％.验证试验结果显示,在此条件下该菌发酵液的干重为4.725 g/L,较优化前增加了31.35％,LD50为0.768％,较优化前降低了25.14％.研究结果为杀藻活性物质以及杀藻机理的研究奠定了理论基础.
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