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Optimization of Catalytic Synthesis of α-hydroxyphenylacetic Acid with Immobilized Cells by Using Response Surface Methodology
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Applied & Environmental Biology
- Issue:
- 4
- DOI:
- 10.3724/SP.J.1145.2012.00667
- Key Word:
- Plackett-Burman;响应面分析法;固定化细胞;α-羟基苯乙酸;微生物转化;催化合成
Abstract： 采用Plackett-Burman试验设计法及响应面分析法(RSM),对固定化Bacillus sp.ULi-11菌株生物不对称合成(R)-α-羟基苯乙酸((R)-HPA)进行优化首先采用Plackett-Burman试验设计筛选影响产率的主要因素,再用最陡爬坡方法逼近最大响应区域后用Box-Behnken试验设计及RSM进行回归分析,得到的最佳条件为温度33℃,增殖时间9.3h,装液量18 mL,转速180 r/min,pH 7.2,接种量2％,种龄16h,在此条件下(R)-HPA的产率为49.61％,比优化前的产率提高了23.7％.
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