You Position: Home > Paper
Phosphorylation of Yeast Protein Kinase Sch9 Regulates Heat Stress Response
( views:37, downloads:0 )
- Author:
- No author available
- Journal Title:
- Chinese Journal of Applied & Environmental Biology
- Issue:
- 3
- DOI:
- 10.3724/SP.J.1145.2012.00369
- Key Word:
- Sch9;热胁迫;磷酸化;PDK1位点;酵母
Abstract： 在酵母(Saccharomyces cerevisiae)中,蛋白激酶Sch9已经被证明与细胞大小、胁迫应答、自噬以及寿命的调控有关.通过不同基因型酵母在固体培养基上的热敏感性研究,发现Sch9的激酶功能与热胁迫相关,进一步通过Sch9特异磷酸化位点抗体的免疫印迹,检测了细胞内Sch9关键的激活环T570位点(PDK1位点)在热胁迫条下的磷酸化状态,发现半乳糖诱导表达的Sch9在热胁迫条件下T570位点去磷酸化,首次证明了Sch9通过调控激酶活性参与细胞热胁迫应答.研究结果揭示了Sch9在胁迫应答中的部分功能,并为生理条件下Sch9调控功能的研究提供了生物化学证据.
- [1]刘军,闾磊,朱德燕,等.酵母（Saccharomyces cerevisiae）蛋白激酶SCH9激活环磷酸化调控胁迫应答[J].2010,(1).
- [2]苗敏,曹红平,钟彦,等.出芽酵母Saccharomyces cerevisiae转录抑制因子Rdr1负调控细胞胁迫应答[J].2009,(12).
- [3]Amy, Trott,Lance, Shaner,Kevin A, Morano.The molecular chaperone Sse1 and the growth control protein kinase Sch9 collaborate to regulate protein kinase A activity in Saccharomyces cerevisiae.[J].2005,170.
- [4]Knighton, D.R.,Zheng, J..Structure of a peptide inhibitor bound to the catalytic subunit of cyclic adenosine monophosphate.. (cover story)[J].1991,253(5018).
- [5]Morano KA,Thiele DJ.The Sch9 protein kinase regulates Hsp90 chaperone complex signal transduction activity in vivo.[J].1999,18(21).
- [6]D R, Alessi,M, Andjelkovic,B, Caudwell,etc.Mechanism of activation of protein kinase B by insulin and IGF-1.[J].1996,15.
- [7]Knighton, D.R.,Zheng, J..Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein... (cover story)[J].1991,253(5018).
- [8]Burtner CR,Kennedy BK,Kaeberlein M,etc.A molecular mechanism of chronological aging in yeast.[J].2009,8(8).
- [9]Cameroni E,Uotila A,Wanke V,etc.Caffeine extends yeast lifespan by targeting TORC1.[J].2008,69(1).
- [10]Uotila A,Bodenmiller B,Huber A.Characterization of the rapamycin-sensitive phosphoproteome reveals that Sch9 is a central coordinator of protein synthesis.[J].2009,23(16).
- [11]M, Johnston,R W, Davis.Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.[J].1984,4.
- [12]Biondi RM.Phosphoinositide-dependent protein kinase 1, a sensor of protein conformation [Review][J].2004,29(3).
- [13]Soulard A,Deloche O,Wanke V,etc.Sch9 is a major target of TORC1 in Saccharomyces cerevisiae[J].2007,26(5).
- [14]Kobayashi T,Cohen P.Activation of serum- and glucocorticoid-regulated protein kinase by agonists that activate phosphatidylinositide 3-kinase is mediated by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and PDK2.[J].1999,339(Pt.2).
- [15]T, Toda,S, Cameron,P, Sass,etc.SCH9, a gene of Saccharomyces cerevisiae that encodes a protein distinct from, but functionally and structurally related to, cAMP-dependent protein kinase catalytic subunits.[J].1988,2.
- [16]T, Hunter.Signaling--2000 and beyond.[J].2000,100(1).