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Optimization of Fermentation and Culture Conditions of Umbelopsis isabellina Hua2-1 Producing Lipid
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Applied & Environmental Biology
- Issue:
- 1
- DOI:
- 10.3724/SP.J.1145.2012.00080
- Key Word:
- 深黄伞形霉华2-1;微生物油脂;发酵条件优化
Abstract： 为开发微生物油脂资源,从富含油脂的土样中分离获得一株产油脂微生物——深黄伞形霉(Umbelopsis isabellina)华2-1,油脂含量达48.60％.采用单因素和正交实验方法,对深黄伞形霉华2-1的发酵培养条件进行优化研究.优化的培养条件为:葡萄糖100 g/L,酵母粉3 g/L,接种量为20％,初始pH值为5.0～6.0,MgSO4·7H2O和KH2PO4的添加浓度分别为0.5 g/L和2 g/L,培养温度为31℃,最佳发酵培养时间为168 h.华2-1在优化发酵条件下可获得菌体生物量、油脂产量和油脂含量分别为45.86 g/L、24.47 g/L和59.53％,较优化前分别提高了21.48％、33.42％和22.49％.因此,深黄伞形霉华2-1作为微生物油脂生产的新资源具有广阔的应用前景.
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