You Position: Home > Paper
Screening of a Thermostable Cellulase-producing Strain and Its Enzymatic Properties
( views:20, downloads:0 )
- Author:
- No author available
- Journal Title:
- Chinese Journal of Applied & Environmental Biology
- Issue:
- 1
- DOI:
- 10.3724/SP.J.1145.2012.00075
- Key Word:
- 耐热纤维素酶;菌株筛选;放线菌;系统发育分析;裂孢菌属;酶学性质
Abstract： 采用纤维素刚果红平板法从大田蘑菇种植场建堆的稻草样中分离得到了1株能产具有较好温度耐受性和pH稳定性的纤维素酶的放线菌DY3.综合形态、生理生化特征以及16S rDNA序列分析,将其初步鉴定为嗜热裂孢菌(Thermobifida fusca).对该菌所产纤维素酶的性质研究表明:最适催化温度为65℃,在70℃保温60 min后仍有75％以上的活力；最适催化pH为7.5,在pH 5.5～10.0之间该酶的稳定性较好,pH 10.0的条件下仍有80％的活力；最适作用底物是羧甲基纤维素钠.研究可为木质纤维素的生物预处理提供一定参考价值.
- [1]G. L. Miller.Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar[J].1959,31(3).
- [2]S. Ito,Tohru Kobayashi,Katsutoshi Ara,etc.Alkaline detergent enzymes from alkaliphiles: enzymatic properties, genetics, and structures[J].1998,2(3).
- [3]Puranen T,Kallio J,Viikri L,etc.Cloning, expression, and characterization of novel thermostable family 7 cellobiohydrolases[J].2008,101(3).
- [4]Barness G,Doyle J,Steinberger Y,etc.Cellulase dynamics in a desert soil.[J].2006,38(2).
- [5]魏艳红,刘德立,熊鹰,等.纤维素酶产生菌HS-F9的筛选鉴定和产酶条件优化[J].2010,(2).
- [6]Jorge JA,Rechia CGV,Rizzatti ACS,etc.Purification and properties of a thermostable extracellularbeta-D-xylosidase produced by a thermotolerant Aspergillus phoenicis[J].2001,26(3).
- [7]F. Will,Katri BauckhageHelmut Dietrich.Apple pomace liquefaction with pectinases and cellulases:analytical data of the corresponding juices[J].2000,211(4).
- [8]Lebeault JM.,Park JH.,Park SH.,etc.Expression of carboxymethylcellulase on the surface of Escherichia coli using Pseudomonas syringae ice nucleation protein[J].1998,22(5).
- [9]P, Béguin,J P, Aubert.The biological degradation of cellulose.[J].1994,13(1).
- [10]Paula Sa-Pereira,Maria Raquel Aires Barros,Maria Costa-Ferreira,etc.Rapid production of thermostable cellulase-free xylanase by a strain of Bacillus subtilis and its properties[J].2002,30(7).
- [11]Govindarajan, S,Heinzelman, P,Snow, CD,etc.A family of thermostable fungal cellulases created by structure-guided recombination[J].2009,106(14).
- [12]Li YH,Ding M,Xu GJ,etc.A novel thermoacidophilic endoglucanase, Ba-EGA, from a new cellulose-degrading bacterium, Bacillus sp.AC-1[J].2006,70(4).
- [13]M.K.Bhat.Cellulases and related enzymes in biotechnology[J].2000,18(5).
- [14]谢天文,袁月祥,闫志英,等.一株嗜酸性产纤维素酶真菌的特性及产酶条件优化[J].2010,(6).