You Position: Home > Paper
In vitro Characteristics of the Cellulases from Reticulitermes chinensis Snyder
( views:9, downloads:0 )
- Author:
- No author available
- Journal Title:
- Chinese Journal of Applied & Environmental Biology
- Issue:
- 1
- DOI:
- 10.3724/SP.J.1145.2012.00070
- Key Word:
- 黑胸散白蚁;纤维素酶;内切β-1,4-葡聚糖酶;纤维二糖水解酶;β-葡萄糖苷酶;酶活;Native-PAGE
Abstract： 采用DNS法研究了我国广泛分布的一种低等木食性白蚁——黑胸散白蚁纤维素酶的体外酶活特性以了解其纤维素降解机制.结果表明,内切β-1,4-葡聚糖酶(EG)、纤维二糖水解酶(CBH)和β-葡萄糖苷酶(BG)这3种酶的最佳反应时间均为15 min,最佳底物浓度为1％,最适反应pH为5.6,最适反应温度为35℃.在最适反应条件下,EG、CBH和BG的活性分别达到71.3(±13.9) U/mg、5.8(±0.8) U/mg和4.1(±0.7) U/mg.EG在体外的热稳定性较差,在50℃及更高温度酶活很低或完全失活,但该酶对pH稳定性较好,在pH 3.2～8.0范围内酶活力变化不大.Native-PAGE电泳检测到该白蚁体内至少有8种不同的EG活性条带,肠道不同部位纤维素酶活性条带种类不同.这些研究表明,木食性白蚁降解纤维素是一个复杂的过程,需要多种纤维素酶的共同作用.
- [1]杨天赐,莫建初,程家安,等.黄胸散白蚁纤维素酶的研究[J].2003,(6).
- [2]刘韫滔,禤淑霞,龙传南,等.纤维素降解菌L-06的筛选、鉴定及其产酶条件的分析[J].2008,(6).
- [3]H, Watanabe,H, Noda,G, Tokuda,etc.A cellulase gene of termite origin.[J].1998,394(6691).
- [4]Ohkuma M..Termite symbiotic systems: efficient bio-recycling of lignocellulose [Review][J].2003,61(1).
- [5]MIURA TORU,MATSUMOTO TADAO,FUJITA AI.Differences in cellulose digestive systems among castes in two termite lineages[J].2008,33(1).
- [6]G, Tokuda,N, Lo,H, Watanabe,etc.Metazoan cellulase genes from termites: intron/exon structures and sites of expression.[J].1999,1447.
- [7]Aurélien, Tartar,Marsha M, Wheeler,Xuguo, Zhou,etc.Parallel metatranscriptome analyses of host and symbiont gene expression in the gut of the termite Reticulitermes flavipes.[J].2009,2.
- [8]Zhang, M.,Wang, Q.,Xie, L.,etc.Microbiome of fungus-growing termites: A new reservoir for lignocellulase genes[J].2011,77(1).
- [9]T. K. Ghose.Measurement of cellulase activities[J].1987,59(2).
- [10]Dunhua, Zhang,Alan R, Lax,John M, Bland,etc.Characterization of a new endogenous endo-β-1,4-glucanase of Formosan subterranean termite (Coptotermes formosanus).[J].2011,41(4).