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Optimization of Aspergillus flavus Culture Conditions and Extraction of Aflatoxin B1
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY
- Issue:
- 5
- DOI:
- 10.3724/SP.J.1145.2010.00724
- Key Word:
- 黄曲霉;黄曲霉毒素B1;大米培养基;温度;湿度;优化
Abstract： 为提取高浓度的黄曲霉毒素B1,以大米为培养基培养黄曲霉,分别在不同温度梯度(15℃、20℃、25℃、30℃、35℃)和湿度梯度(0%、80%、85%、90%和95%和100%)下培养.每天测量一次菌斑直径的变化(共测18次),然后采用Logistic(一级)和Ratkowsky(二级)模型对黄曲霉菌斑大小进行拟合和检验,最后借助响应面模型对黄曲霉产毒条件进行优化.结果显示,在35℃和100%湿度的培养条件下,只需培养16～17 d即可获得直径较大的黄曲霉菌斑,提取黄曲霉毒素B.浓度为3 312 ng/mL,单位面积黄曲霉毒素B1产量是优化前(1 101.471 ng/mL)的3倍.
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