Abstract： Interspecific relationship is an important factor affecting community composition, structure, function and dynamics. In order to understand the interspecific segregation in Alniphyllum fortunei forest, 92 quadrats were investigated and a distribution map was made for all trees with H≥2 m and species numbers＞8. Based on the data, using the coefficient of segregation by the subtable of the N×N nearest-neighbor contingency table, the interspecific segregations of the tree species in A. Fortunei forest in the Meihuashan Nature Reserve in Hujian, China were studied. The result indicated that most of species pairs were positively segregated (73.53%), only a few species pairs were randomly segregated (26.47%), no negatively segregated pairs were found. Large individuals that were subdominant species in the forest tended to be positively segregated with other large trees, due to their strong capacity in adaptation and competition. In addition, a new concept, that is, the overall segregation was introduced. The overall segregated pattern in all species in the forest was studied by χ~2 test based on the N×N nearest-neighbor contingency table. The result showed that 17 studied species were overlapped in distribution and characterized by the overall non-segregation.