You Position: Home > Paper

Construction of Twin T-DNA Vector Encoding Hairpin RNA Targeting the Replicase Gene of Barley Yellow Dwarf Virus

( views:167, downloads:0 )
Author:
No author available
Journal Title:
CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY
Issue:
6
DOI:
10.3724/SP.J.1145.2008.00745
Key Word:
大麦黄矮病毒BYDV;RNAi;双T-DNA区载体;无选择标记基因植物;barley yellow dwarfvirus(BYDV);RNAi;twin T-DNAs vector;marker-free plant

Abstract: RNA interference(RNAi)technology has become the most effective approach for genetically engineering virus resistance in plants.Here,we constructed a hairpin RNA(hpRNA)-expressing RNAi vector targeting the replicase gene of a Chinese isolate of barley yellow dwarf virus(BYDV),BYDV-GAV.This construct was comprised of two tandem T-DNAs (twin T-DNAs),one of which contained the hygromyein resistance gene hpt as the selectable marker,and the other contained the expression cassette encoding hpRNA of the polymerase gene sequence of BYDV-GAV.This twin T-DNA construct should allow the production of selectable marker-free transgenic plants that are resistant to BYDV-GAV.Fig6,Ref21

WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn