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Field Degradation Dynamics of Cry1Ab Insecticidal Protein in Transgenic Bacillus thuringiensis Corn Pollen Accumulated in Leaf Axiles
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY
- Issue:
- 2
- DOI:
- No doi available
- Key Word:
- 转Bt基因玉米;花粉;Cry1Ab杀虫蛋白;叶腋;降解
Abstract： 以表达Cry1Ab杀虫蛋白的两种转Bt基因抗虫玉米MON810和Bt11为材料,采用ELISA方法研究了玉米散粉时沉积在植株叶腋处Bt玉米花粉中Cry1Ab杀虫蛋白的田间降解动态,比较了两种Bt玉米花粉中Cry1Ab杀虫蛋白的降解速度.结果表明, Bt玉米花粉中Cry1Ab杀虫蛋白是逐渐降解的,且降解速度逐渐加快.但两种Bt玉米花粉中Cry1Ab杀虫蛋白降解速度不同, MON810中的Bt杀虫蛋白降解较快,而Bt11中的降解较慢. D 15时在MON810叶腋处的花粉中已经检测不到Bt杀虫蛋白,而在Bt11花粉中Bt杀虫蛋白到d 18才完全降解.两种Bt玉米花粉中杀虫蛋白的初始含量差异显著,且在各个取样时间内Bt杀虫蛋白的残留量存在显著差异.玉米散粉时留在田间的Bt玉米花粉中的Cry1Ab杀虫蛋白不会在自然界累积.
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