You Position: Home > Paper
Transformation of Phytase Gene into Rice by Particle Bombardment
( views:219, downloads:0 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY
- Issue:
- 1
- DOI:
- 10.3321/j.issn:1006-687X.2008.01.002
- Key Word:
- 水稻;植酸酶基因;遗传转化;植酸酶
Abstract： 植酸酶是一类能促进植酸及其盐类水解成肌醇和磷酸的酶的总称.它能提高饲料和食品中磷的利用率,减轻粪便中磷排放所造成的环境污染,清除植酸与金属离子的螯合作用,改善微量元素的吸收和利用.本研究将马铃薯的胞外分泌信号肽(Psec)和大肠杆菌的植酸酶基因(appA2)结合组成融合基因导入粳稻台北309中.PCR检测和Southern杂交表明,植酸酶基因和分泌信号肽序列已经整合到水稻的基因组中.无机磷含量分析表明,含目的基因的转基因水稻种子及茎叶中的无机磷含量均较未转化植株有显著的提高.图6表1参14
- [1]郝建国,李晶,黄萱,等.用基因枪介导法将水稻几丁质酶基因导入小麦[J].2004,(4).
- [2]胡光珍,王幼芳,何玉科.转基因植物对有机污染物的吸收、转化和降解[J].2005,(4).
- [3]李钱峰,刘巧泉,张达江,等.转基因水稻中重组植酸酶的表达[J].2006,(3).
- [4]D M, Denbow,E A, Grabau,G H, Lacy,etc.Soybeans transformed with a fungal phytase gene improve phosphorus availability for broilers.[J].1998,77.
- [5]鲍士旦.土壤农化分析[M].2007.
- [6]S, Sebastian,S P, Touchburn,E R, Chavez,etc.The effects of supplemental microbial phytase on the performance and utilization of dietary calcium, phosphorus, copper, and zinc in broiler chickens fed corn-soybean diets.[J].1996,75.
- [7]K H, Brown,N W, Solomons.Nutritional problems of developing countries.[J].1991,5(2).