Genetic Variation of Hippophae rhamnoides Populations at Different Altitudes in the Wolong Nature Reserve Based on RAPDs
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY
- Issue:
- 6
- DOI:
- 10.3321/j.issn:1006-687x.2007.06.001
- Key Word:
- 海拔梯度;遗传分化;遗传变异;沙棘;RAPD;altitudinal gradient;genetic differentiation;genetic variability;Hippophae rhamnoides;RAPD
Abstract： To estimate genetic variation and genetic structure of Hippophae rhamnoides ssp. sinensis at different altitudes, five natural populations in the Wolong Nature Reserve at altitudes ranging from 1 800 to 3 400 m above sea level ( a. s. l. ) were surveyed using random amplified polymorphic DNA markers (RAPDs). Based on 11 decamer primers, a total of 151 reproducible DNA loci were yielded, and 143 of them were polymorphic, accounting for 94.7%. Among the five populations investigated, the total gene diversity (HT) and gene diversity within population B equaled 0. 289 and 0. 315, respectively, which are modest for a subspecies of H. rhamnoides that is an outcrossed and long-lived woody plant. The amount of genetic variation among populations varied from 0.098 within population E (3 400 ma. s. l. ) to 0. 315 within population B (2 200 ma. s. l. ).The coefficient of gene differentiation (GST) among populations equaled 0.406, and revealed that 40.6% of the genetic variance existed among populations and 59.4% within populations. The population A ( 1 800 ma. s. l. ) differed greatly from the other four populations, which contributes to high genetic differentiation. A UPGMA cluster analysis and principal coordinate analyses based on Nei's genetic distances further corroborated the relationships among the five populations and all the sampling individuals, respectively. Mantel tests detected a significant correlation between genetic distances and altitudinal gradients (r=0.646, P=0.011). Fig3, Tab4, Ref42
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