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Construction of Wheat Translation Initiation Factor 5A (eIF5A) Expression Vector and Its Prokaryotic Expression
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY
- Issue:
- 3
- DOI:
- 10.3321/j.issn:1006-687X.2007.03.003
- Key Word:
- 小麦;蛋白翻译起始因子5A;原核表达;Western blot
Abstract： 真核生物的翻译起始因子5A(eIF5A)是调控生物生长发育、衰老及环境适应等的重要因子.在对小麦eIF5A基因进行克隆和序列分析的基础上,成功构建了小麦蛋白翻译起始因子5A(eIF5A)的表达载体pET28a(+)-eIF5A,并且经IPTG的诱导进行了原核表达,结果表明,eIF5A能够体外高效表达;进一步的Western blot分析表明,所表达的约18×103特异蛋白确为小麦eIF5A蛋白.
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