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Comparison of RNA Silencing Inducing Efficiency of Three PVX CP Gene Constructs via Transient Expression

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Author:: No author available

Journal Title:: CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY

Issue:: 1

DOI:: 10.3321/j.issn:1006-687X.2007.01.001

Key Word:: RNA沉默;马铃薯X病毒(PVX);外壳蛋白(CP)基因;农杆菌渗入;抗性

Abstract： RNA沉默是植物抵御病毒侵染的一种防卫机制,病原来源的抗性(pathogen-derived resistance, PDR)被认为是通过RNA沉默起作用的.转化的核酸片段在基因组中的位置、长短和不同排列结构等均影响RNA沉默的效率.用全长马铃薯X病毒(PVX) CP基因构建非翻译的正义、反义和反向重复结构转基因的植物表达载体,通过农杆菌渗入法瞬时表达测试了这些转基因对RNA介导抗性的诱导效率和有效性.结果表明,反向重复的PVX CP是更为有效的RNA沉默诱导因子,同时也证明让目的基因在受试植物中瞬时表达,在转化植物之前能比较快速地检测转基因的表达情况以及表达产物的功能,从而节约时间和资源,并减少盲目性.图3 表2 参21

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