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Cloning and Sequencing of Nucleotide-binding Site (NBS)-Leucine-rich Repeats (LRR) Region of Cereal Cyst Nematode Resistance Gene
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY
- Issue:
- 6
- DOI:
- 10.3321/j.issn:1006-687X.2006.06.001
- Key Word:
- 易变山羊草;抗禾谷孢囊线虫基因;核苷酸结合位点区-亮氨酸重复序列区;克隆和序列分析
Abstract： 根据源于节节麦抗禾谷孢囊线虫基因Cre3及已经克隆的NBS-LRR类植物抗病基因的NBS与LRR区保守序列分别设计特异引物,从易变山羊草基因组中PCR扩增得到两个扩增条带,它们的大小分别约为530 bp和1 200 bp.经克隆测序发现,这两个序列分别长为532 bp和1 175 bp,且是连续的.它们有32 bp的共同序列,总长为1 675 bp,包含了一个NBS-LRR区和一个不完整的开放阅读框,没有起始密码子、终止密码子和内含子结构.它编码一个557个氨基酸的蛋白质,分子量(Mr)为63.537×103,含有NBS区保守模体ILDD, ESKILVTTRSK, KGSPLAARTVGG, RRCFAYCS, EGF,以及LRR区的保守模体aXXLXXLXXL.它与Cre3的核苷酸和氨基酸同源性分别为87.8%和77%,可能是一个抗禾谷孢囊线虫基因. 图6 参13
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