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Differences of Gene Expression in Mycelia of Lentinula edodes under Cold Stress
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY
- Issue:
- 5
- DOI:
- 10.3321/j.issn:1006-687X.2006.05.004
- Key Word:
- 香菇;低温胁迫;mRNA差异显示;基因表达序列标签(ESTs)
Abstract： 香菇为变温结实型担子菌,为探讨低温胁迫诱导香菇子实体形成的分子机理,应用mRNA差异显示技术对低温胁迫下香菇菌丝体基因表达差异进行了比较.电泳差异展示结果表明,低温胁迫前后基因表达在数量水平和质量水平上都存在显著差异,数量水平的差异表现为低温胁迫过程中表达序列标签(expressed sequence tags,ESTs)增强表达或减弱表达;质量水平上的差异表现为低温胁迫使部分ESTs特异诱导表达或沉默表达.差异ESTs的类型比较和多态性分析发现,低温胁迫处理6～12 h可能是诱发香菇原基发生的关键时段.本文还对显著差异表达的基因片段进行序列分析和Northern杂交验证,初步讨论了低温胁迫时间在调控香菇子实体发生中的作用.图4表2参13
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