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Isolation and Characterization of a DRE-Binding Transcription Factor Gene from Festuca arundinacea Schreb.
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY
- Issue:
- 2
- DOI:
- 10.3321/j.issn:1006-687X.2006.02.001
- Key Word:
- 高羊茅;DRE元件;转录因子;胁迫
Abstract： 为了鉴定草坪草高羊茅(Festuca arundinacea Schreb.)中与低温、高盐和干旱胁迫相关的基因,我们构建了冷诱导(4℃)的高羊茅cDNA文库,并从这个文库中分离得到一个DREB类转录因子基因,FaDREB1A.序列分析表明,FaDREB1A具有一个717 bp的开放阅读框和143 bp的3'非编码区,推测的氨基酸序列中含有一个高度保守的EREBP/AP2结构域.酵母单杂交结果表明,FaDREB1A蛋白能在体外特异结合DRE元件,并具有转录激活功能.Southern杂交结果显示,FaDREB1A在高羊茅基因组中可能是单拷贝或低拷贝基因.Northern杂交结果发现,FaDREB1A基因受低温、高盐和干旱的诱导表达,对ABA没有反应,说明FaDREB1A基因在高羊茅植株对低温、高盐和干旱的应答反应中起重要作用.图5参30
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