Abstract： Kunming mice (KM) were treated with geniposide and with CCl4. Then, the hepatic protective effect of geniposide was thus studied by measuring the activities of serum ALT and AST, the content of GSH of the mice by histopathological examination. The results showed that geniposide could suppress the increase of ALT and AST, and decrease GSH. In order to understand the possible biochemical mechanism of this effect, normal mice were treated with different concentrations of geniposide,and then the activity of CYP450 2E1 in the liver microsome and those of GST and GR in liver were assayed. After the mice were treated with 50 mg/kg, 100 mg/kg or 150 mg/kg of geniposide once a day for 5 days, the activity of CYP450 2E1 decreased and those of GST and GR increased significantly compared with the control groups. These results suggested that geniposide protect hepatic damage by decreasing the activities of CYP450 2E1 and increasing those of GR and GST, all of which are closely related to radical elimination. Fig 1, Tab 3, Ref 16