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COLONY FORMATION AND SELENIUM-ENRICHED STABILITY OF SPIRULINA PLATENSIS UNDER SELENIUM STRESS
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY
- Issue:
- 5
- DOI:
- 10.3321/j.issn:1006-687X.2005.05.005
- Key Word:
- 钝顶螺旋藻;集落化;硒
Abstract： 探讨在琼脂糖半固体培养基中硒胁迫螺旋藻(Spirulina platensis,SP)集落形成及其富硒稳定性.培养基中添加亚硒酸钠,硒含量分别为2.5 mmol L-1、5 mmol L-1和10 mmol L-1,培养并观察螺旋藻集落形成,挑取藻集落进行富硒培养,多次转种后,测定生长率、硒含量,筛选稳定的富硒螺旋藻.从含硒5 mmol L-1的培养皿中挑选12个典型藻丝集落接种培养,发现其中6个生长率达到对照的80%,有两个藻集落(编号为SP0803和SP0809)硒含量较高,干藻硒含量分别为p(Se)=924μg/g和p(Se)=831μg/g,连续3次转种培养,藻体含硒量保持稳定.以上结果表明,利用琼脂糖半固体培养基进行微藻集落培养是可行的,并成功筛选到富硒螺旋藻.图3表2参12
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